提高小麦基因枪法转化频率的研究

用基因枪法将带Ｂａｒ－ＧＵＳ双标记基因的质粒较入普通春小麦品种中－６０６３４的幼胚盾片，并获基因植株。在轰击的经预培养３－４天的３４２块幼胚片再经筛选再生的植株中，经ＰＣＲ和Ｓｏｕｔｈｅｒｎ分析表明，

Studies of Improving the Frequency of Wheat Transformation by Biolistic Bombardment

In the present study immature embryos of spring wheat Zhong-60634 were bombarded with gold particles coated with pAHC25 containing Bar and GUS genes. A total of 17 bialaphos-tolerant plants were obtained from 342 immature embryos which were first cultured for 3-4 days and then bombarded and selected. Integration of the introduced genes into the genome of bosgenic wheat plants was shown by PCR and Southern analysis and transformation frequency was 0.88%. Furthermore, the present study showed that calli regeneration ability was improved while the embryo culture medium was supplemented with 2mg/L dicamba instead of 2mg/L 2,4-D. Meanwhile,in order to keep the calli differentiation ability calli should be transfered to differentiation meditum supplemented with 3mg / L bialaphos from calli selection medium with 5mg/L bialaphos within a month.