| 柑桔原生质体融合再生叶肉亲本型植株的遗传分析 |
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| 引用本文： | 史永忠,邓秀新.柑桔原生质体融合再生叶肉亲本型植株的遗传分析[J].遗传学报,1999,26(3):244-248. |
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| 作者姓名： | 史永忠 邓秀新 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室柑桔研究所!武汉，430070，华中农业大学作物遗传改良国家重点实验室柑桔研究所!武汉，430070 |
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| 基金项目： | 国家自然科学基金!39330150 |
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| 摘 要： | 叶肉亲本（粗柠檬）型植株叶形指数、气孔特征与亲本粗柠檬无异，与同组合体细胞杂种差异显著。染色体计数为二倍体（２ｎ＝２ｘ＝１８）。过氧化物酶（ＰＯＸ）、多酚氧化酶（ＰＰＯ）、谷草转氨酶（ＧＯＴ）同工酶图谱与粗柠檬一致。ＲＡＰＤ分析表明，在具多态性的５４个随机引物中，大多数引物（５２个）上的图谱与粗柠檬相同。但ＯＰＷ－１２上，叶肉亲本型植株含有哈姆林甜检的特征谱带。ＯＰＶ－０４扩增产物显示，叶肉亲本型
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| 关 键 词： | 柑桔 体细胞融合 再生植株 RAPD |
Genetic Analysis of Leaf-parent-type Plants Regenerated from Somatic Hybridization in Citrus |
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| SHI Yong-Zhong, DENG Xiu-Xin.Genetic Analysis of Leaf-parent-type Plants Regenerated from Somatic Hybridization in Citrus[J].Journal of Genetics and Genomics,1999,26(3):244-248. |
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| Authors: | SHI Yong-Zhong DENG Xiu-Xin |
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| Institution: | State Key Laboratory in Genetic Improvement of Crops and Citrus Research Institute Huazhong Agricultural University Wuhan 430070 |
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| Abstract: | The leaf indexes and stoma characteristics of leaf-parent-type regenerants were the same as those of the leaf parent Rough lemon, but quite different from the somatic hybrids. Leaf-Parent-type plants were diploid (2n=2x=18). Patterns of POX, PPO, GOT isozymes were identical to those of Rough lemon. The plants were analyzed by using 54 arbitrary primers with polymorphisms. Most of the primers (52) showed that leaf-parent-type plants were in concordance with Rough lemon. However, with primer OPW-12, the plants contained a unique band of Hamlin sweet orange. Amplification products with primer OPV-04 showed slight differences among the accessions. The research suggested that most genetic constitutions of the leaf-parent-type plants were from leaf parent Rough lemon and a little from embryogenic suspension parent Hamlin sweet orange. |
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| Keywords: | Citrus Somatic hybridization Plant regeneration Random amplified polymorphic DNA (RAPD) |
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