猪单个精子单倍型分析方法研究

应用引物延伸预扩增，内嵌引物设计策略进行了ＰＣＲ扩增并结合聚丙烯酰胺凝胶电泳及银染技术，对猪单个精子１２号染色体上４个微卫星位点进行了ＰＣＲ扩增。结果表明，上述技术的应用可以清晰地鉴定出每个精子的单倍型，单精子分型技术的应用为猪高精度遗传作图及需要样本量足够的大遗传现象研究提供了独特的工具。

Haplotype Identification of Single Sperm in Pigs

Haplotype of porcine single sperm was analyzed by PCR method through using primer extension preamplification and heminesting primer design strategy. The PCR products were run on 8% polyacrylamide gel, and visualized by silver staining method.The results showed that the haplotypes of individual sperm can be unequivocally demonstrated by these methods. The application of single sperm typing provides a unique tool for the construction of high resolution genetic map and the study of genetic phenomena requiring large sample size.