| 两个可进入种系的ES细胞系的建立 |
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| 引用本文： | 韩嵘,孟令国.两个可进入种系的ES细胞系的建立[J].遗传学报,1999,26(3):208-212. |
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| 作者姓名： | 韩嵘 孟令国 |
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| 作者单位： | [1]北京大学生命科学学院 [2]北京大学校医院 |
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| 基金项目： | 国家科委863高技术研究发展计划资助，国家自然科学基金 |
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| 摘 要： | 从１２９／ｔｅｒ小鼠中建立了９个ＥＳ细胞系，它们在核型、生长速度、体内外分化能力等方面显示了各自不同的特点。通过囊胚显微注射法，将ＥＳ细胞注入Ｃ５７ＢＬ／６Ｊ胚胎中，制作了嵌合体，并通过对嵌合体后代毛色的观察，判断了嵌合体生殖细胞的组成。结果表明，ＥＳ细胞系ＭＥＳＰＵ２１、ＭＥＳＰＵ２２都具有很强的种系嵌合能力。比较这两个细胞系与其他细胞系，证明一个好的ＥＳ细胞系必须具备核型正常、生长速度快、体外
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| 关 键 词： | 小鼠 ES细胞系 种系嵌合 |
Establishment of Two ES Cell Lines with Good Germline Contribution |
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| HAN Rong, CHEN Wei-Sheng, TONG Ying, SHANG Ke-Gang.Establishment of Two ES Cell Lines with Good Germline Contribution[J].Journal of Genetics and Genomics,1999,26(3):208-212. |
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| Authors: | HAN Rong CHEN Wei-Sheng TONG Ying SHANG Ke-Gang |
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| Institution: | HAN Rong; CHEN Wei-Sheng; TONG Ying; SHANG Ke-Gang(College of Life Sciences Peking University Beijing 100871)MENG Ling-Guo(peking University Hospital Beijing 100871) |
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| Abstract: | Nine embryonic stem cell lines have been established from mouse strain 129/ter. Three of the nine ES cell lines were karyotypically normal. The nine cell lines showed some difference in the growth rate and the differential competence. Chimeras were made by injecting the ES cells into C57BL/ 6J blastocysts, and the germline compositions of the chimeras were detected by mating them with albino ICR mice. The results indicated that ES cell line MESPU21 and MESPU22 were both highly germline-competent. Comparing with other ones, these tWo cell lines both were karyotypically normal and propagated fast. The tissue composition of the teratocarcinomas derived from these cell lines appeared paralle to the results of chimera production. Careful manipulation during the process of ES cell establishment was helpful to obtain good cell lines. |
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| Keywords: | Mouse ES cell line Germline chimeras |
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