| 内蒙古白绒山羊4E-BP1基因cDNA克隆及组织表达特异性分析 |
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| 引用本文： | 傅海霞,杨娇馥,梁燕,陈宇浩,王志钢. 内蒙古白绒山羊4E-BP1基因cDNA克隆及组织表达特异性分析[J]. 生物技术通报, 2012, 0(3): 90-94 |
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| 作者姓名： | 傅海霞 杨娇馥 梁燕 陈宇浩 王志钢 |
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| 作者单位： | 内蒙古大学生命科学学院,呼和浩特,010021 |
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| 摘 要： | 旨在克隆内蒙古白绒山羊4E-BP1(真核细胞翻译起始因子4E结合蛋白1)基因并进行生物信息学及表达模式分析。根据已报道物种4E-BP1基因cDNA序列,用primer premier5软件设计引物,通过RT-PCR从绒山羊胎儿成纤维细胞总RNA中扩增出4E-BP1基因编码区cDNA序列,对目的片段进行测序及表达模式分析。克隆到的内蒙古白绒山羊4E-BP1基因cDNA全长357 bp,包含了完整的的ORF,编码118个氨基酸残基。核酸序列与牛、马、人、大鼠及小鼠的同源性分别为98%、90%、90%、88%和87%。4E-BP1基因在绒山羊脑、心脏、睾丸及胰腺组织中均有表达。
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| 关 键 词： | 内蒙古白绒山羊 4E-BP1 组织特异性表达 |
Cloning and Tissue-specific Expression Pattern of 4E-BP1 Gene cDNA in Inner Mongolia Cashmere Goat |
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| Fu Haixia , Yang Jiaofu , Liang Yan , Chen Yuhao , Wang Zhigang. Cloning and Tissue-specific Expression Pattern of 4E-BP1 Gene cDNA in Inner Mongolia Cashmere Goat[J]. Biotechnology Bulletin, 2012, 0(3): 90-94 |
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| Authors: | Fu Haixia Yang Jiaofu Liang Yan Chen Yuhao Wang Zhigang |
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| Affiliation: | Fu Haixia Yang Jiaofu Liang Yan Chen Yuhao Wang Zhigang(College of Life Sciences,Inner Mongolia University,Hohhot 010021) |
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| Abstract: | The present study aims at cloning the CDS fragment of 4E-BP1(eukaryotic translation initiation factor 4E binding protein 1)gene cDNA and analyzing its tissue-specific expression pattern in Inner Mongolia cashmere goat.4E-BP1 gene cDNA was amplified by RT-PCR from Inner Mongolia cashmere goat fetal fibroblasts.The nucleotide sequence was analyzed with bioinformatics.The tissue-specific expression pattern of 4E-BP1 gene was analyzed by semi-quantitative RT-PCR.The cloned 4E-BP1 gene cDNA was 357 bp in length,including a complete ORF encoding 118 amino acids.The full cDNA nucleotide sequence has a 98%,90%,90%,88% and 87% identity with Bos taurus,Equus caballus,Homo sapiens,Rattus norvegicus and Mus musculu.The 4E-BP1 gene was shown to express in brain,heart,testis and pancreas. |
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| Keywords: | Inner Mongolia cashmere goat 4E-BP1 Tissue-specific expression |
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