| 微核技术研究进展 |
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| 引用本文： | 龚婧婧,黄海涛,米其利,倪红梅,季秀玲,夭建华. 微核技术研究进展[J]. 生物技术通报, 2012, 0(3): 49-56 |
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| 作者姓名： | 龚婧婧 黄海涛 米其利 倪红梅 季秀玲 夭建华 |
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| 作者单位： | 1. 昆明理工大学生命科学与技术学院,昆明,650224
2. 云南烟草科学研究院,昆明,650106 |
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| 基金项目： | 云南中烟工业有限责任公司项目,中国烟草总公司项目 |
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| 摘 要： | 微核试验作为检测染色体损伤最常用而有效的细胞遗传学检测方法之一,经济、简便、快速,在敏感性、特异性和准确性方面,与经典的染色体畸变分析方法基本相当。主要综述国内外微核检测技术的最新研究进展,尤其是微核的自动化检测技术。其中流式细胞仪自动化检测和激光扫描细胞仪自动化检测,以及微核试验高内涵筛选方法由于其特有的优势,应用和发展前景广阔。
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| 关 键 词： | 微核 自动化 流式细胞仪 激光扫描细胞仪 高内涵筛选 |
Research Progress on Micronucleus Technology |
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| Gong Jingjing , Huang Haitao , Mi Qili , Ni Hongmei , Ji Xiuling , Yao Jianhua. Research Progress on Micronucleus Technology[J]. Biotechnology Bulletin, 2012, 0(3): 49-56 |
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| Authors: | Gong Jingjing Huang Haitao Mi Qili Ni Hongmei Ji Xiuling Yao Jianhua |
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| Affiliation: | 1Kunming University of Science and Technology,Kunming 650224;2Institute of Yunnan Academy of Tobacco Science,Kunming 650106) |
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| Abstract: | Micronucleus formation is a comprehensive reflection of biological damage caused by a variety of physical and chemical factors.As one of the most common and effective cytogenetic detection methods,micronucleus test detects fast in speed,simple in operation,cheap in cost,with respect to the sensitivity,specificity and accuracy.They are roughly equal with that of the classic analysis of chromosome aberrations.In this article,the latest research progress on micronucleus technology,especially the automated technology were summarized.Thereinto,the automated detection of flow cytometry and laser scanning cytometry are the high throughout screening micronucleus assays,they have a good development and a wide application prospects because of its unique advantages. |
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| Keywords: | Micronucleus Automation Flow cytometry Laser scanning cytometry High content screening |
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