Human umbilical cord blood serum promotes growth, proliferation, as well as differentiation of human bone marrow-derived progenitor cells |
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Authors: | Smruti M Phadnis Mugha V Joglekar Vijayalakshmi Venkateshan Surendra M Ghaskadbi Anandwardhan A Hardikar Ramesh R Bhonde |
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Institution: | (1) Division of Animal Sciences, Agharkar Research Institute, 41004 Pune, India;(2) Tissue Engineering and Banking Laboratory, National Genter for Cell Science, 41107 Pune, India;(3) Stem Cell and Diabetes Section, National Center for Cell Science, 41107 Pune, India;(4) Department of Biochemistry, National Institute of Nutrition, Jamia Osmania, 500007 Hyderabad, India |
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Abstract: | Summary Felal calf serum (FCS) is conventionally used for animal cell cultures due to its inherent growth-promoting activities. However
animal welfare issues and stringent requirements for human transplantation studies demand a suitable alternative for FCS.
With this view, we studied the effect of FCS, human AB serum (ABS), and human umbilical cord blood serum (UCBS) on murine
islets of Langerhans and human bone marrow-derived mesenchymal-like cells (hBMCs). We found that there was no difference in
morphology and functionality of mouse islets cultured in any of these three different serum supplements as indicated by insulin
immunostaining. A comparative analysis of hBMCs maintained in each of these three different serum supplements demonstrated
that UCBS supplemented media better supported proliferation of hBMCs. Moreover, a modification of adipogenic differentiation
protocol using UCBS indicates that it can be used as a supplement to support differentiation of hBMCs into adipocytes. Our
results demonstrate that UCBS not only is suitable for maintenance of murine pancreatic islets, but also supports attachment,
propagation, and differentiation of hBMCs in vitro. We conclude that UCBS can serve as a better serum supplement for growth,
maintenance, and differentiation of hBMCs, making it a more suitable supplement in cell systems that have therapeutic potential
in human transplantation programs. |
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Keywords: | umbilical cord blood serum fetal calf serum islets bone marrow serum supplement |
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