Production of a heterologous endo-1,4-beta-xylanase in the yeast Pichia stipitis with an O(2)-regulated promoter |
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| Authors: | Passoth Hahn-Hägerdal |
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| Affiliation: | a Applied Microbiology, Lund University, P.O. Box 124, 22100 Lund, Sweden |
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| Abstract: | The Cryptococcus albidus XLN-gene (encoding endo-1,4-β-xylanase) was expressed in the yeast Pichia stipitis under the control of the PsADH2-promoter, which is activated under O2 limitation. The resulting transformant produced endo-1,4-β-xylanase after a shift to anoxic conditions. Endo-1,4-β-xylanase production was enhanced by limited aeration after the shift. |
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| Keywords: | Pichia stipitis Heterologous gene expression ADH Endo-1,4-β-xylanase |
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