The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species |
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| Authors: | Ian Godwin Gordon Todd Brian Ford-Lloyd H John Newbury |
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| Institution: | (1) School of Biological Sciences, University of Birmingham, P.O. Box 363, B15 2TT Edgbaston, Birmingham, UK;(2) Present address: Department of Agriculture, University of Queensland, 4067 St Lucia, Qld, Australia |
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| Abstract: | Summary Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dicotyledonous species by strains N2/73 and A281. The presence of acetosyringone during co-cultivation generally enhanced the virulence of these strains, most markedly N2/73 on A. majus and G. max, and A281 on G. max. Strain Ach5 was virulent only on N. tabacum in the absence of acetosyringone, which, when present, extended the host range to include A. majus. There was evidence to suggest that acetosyringone may suppress virulence in some strain/plant species interactions. Virulence was affected in some cases by medium pH, but there was no general effect across plant species.Abbreviations T-DNA
DNA transferred to plant cells by Agrobacterium
- BAP
benzyl aminopurine
- MS medium
Murashige and Skoog (1962) medium |
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| Keywords: | Agrobacterium tumefaciens Allium cepa Antirrhinum majus Brassica campestris Glycine max Nicotiana tabacum transformation |
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