Identification of a Vibrio strain producing antimicrobial agents in the excretory organs of Nautilus pompilius (Cephalopoda: Nautiloidea) |
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Authors: | M. Pernice D. Destoumieux-Garzón J. Peduzzi S. Rebuffat R. Boucher-Rodoni |
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Affiliation: | (1) UMR 5178 CNRS-MNHN-UPMC, Biologie des Organismes Marins et Ecosystèmes, Département Milieux et Peuplements Aquatiques, Muséum National d’Histoire Naturelle, CP 51, 55 rue Buffon, 75005 Paris, France;(2) Laboratoire de Chimie et Biochimie des Substances naturelles, UMR 5154 CNRS-MNHN, Département Régulations, Développement et Diversité Moléculaire, Muséum National d’Histoire Naturelle, CP 54, 57 rue Cuvier, 75005 Paris, France |
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Abstract: | The aim of the present study was to identify and characterize bacteria producing antimicrobial compounds in the excretory organs of Nautilus pompilius. Culture-dependent and culture-independent complementary approaches were used for bacterial identification such as: culture on selective media, Gram staining, CARD-FISH, direct DNA extraction from host tissue, PCR amplification and sequencing of the bacterial 16S rRNA gene. Results show presence of three bacterial groups: γ-Proteobacteria with three clusters (Pseudomonadales, Vibrionales, Alteromonadales), β-Proteobacteria and spirochetes. In order to screen for active strains, antimicrobial activity was tested by diffusion agar assay against Micrococcus luteus, Escherichia coli, Vibrio harveyi, and Candida albicans. Nautilus isolates showed antimicrobial activities against both Gram-positive and Gram-negative reference strains. Most of the active strains were phylogenetically related to environmental Vibrionaceae. These strains were always abundant in N. pompilius PA but were absent from Nautilus macromphalus from other geographical areas. Therefore, we suggest that antimicrobial active Vibrionaceae infect N. pompilius by environmental transmission. |
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Keywords: | 16S rDNA Bacteria Antimicrobial activity Cephalopods Nautilus |
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