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Application of radiation hybrid in gene mapping |
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| Authors: | HE Kaili GU Baiwei ZHANG Qinghua FU Gang WU Jisheng HAN Zeguang CAO Wenjun ZOU Jun MAO Mao LIU Jianxiang CHEN Zhu CHEN Saijuan |
| Affiliation: | (1) Key Laboratory for Human Genome Research and Shanghai Institute of Hematology, Rui-Jin Hospital, Shanghai Second Medical University, 200025 Shanghai, China |
| Abstract: | Radiation hybrid (RH) mapping technique was exploited to determine chromosome locations of 26 human novel full length cDNAs recently cloned. All these cDNA clones were isolated from human cord blood CD + 34 cells and may be related to regulation of hematopoiesis. 23 genes were successfully mapped to chromosomal positions, while RH analyses were not possible in the remaining 3 cases. RH technique is indeed a powerful tool for mapping novel cDNA sequences due to its rapidity, precision, convenience and reproducibility. |
| Keywords: | gene mapping radiation hybrid hematopoietic regulation. |
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