Deficit of CD38/cyclic ADP-ribose is differentially compensated in hearts by gender |
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Authors: | Takahashi Jun Kagaya Yutaka Kato Ichiro Ohta Jun Isoyama Shogen Miura Masahito Sugai Yoshinao Hirose Masanori Wakayama Yuji Ninomiya Mototsugu Watanabe Jun Takasawa Shin Okamoto Hiroshi Shirato Kunio |
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Institution: | Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan. |
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Abstract: | To elucidate whether myocardial CD38/cyclic ADP-ribose (cADPR) signaling plays a physiological role, we investigated the heart of CD38 knockout mice (CD38KO). In CD38KO, the myocardial cADPR content was reduced by 85% compared with wild-type mice (WT). Cardiac hypertrophy developed only in males. At 36 degrees C, none of the parameters for Ca(2+) transients and forces of the papillary muscles differed between WT and CD38KO. In contrast, at 27 degrees C, at which cADPR does not work, the peak Ca(2+)](i) was increased and the decline in Ca(2+)](i) was accelerated in CD38KO compared with WT. In CD38KO, the protein expression of SR Ca(2+) ATPase type2 (SERCA2) and the SERCA2-to-phospholamban ratio were increased compared with WT. The ryanodine receptor protein was increased only in female CD38KO compared with WT. These data suggest that the CD38/cADPR signaling plays an important role in intracellular Ca(2+) homeostasis in cardiac myocytes in vivo. Its deficiency was compensated differentially according to gender. |
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Keywords: | Cyclic ADP-ribose CD38 Calcium cycling Hypertrophy Gender difference |
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