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Natural hybridization origin of <Emphasis Type="Italic">Rhododendron agastum </Emphasis>(Ericaceae) in Yunnan,China: inferred from morphological and molecular evidence
Authors:Jing-Li Zhang  Chang-Qin Zhang  Lian-Ming Gao  Jun-Bo Yang  Hong-Tao Li
Institution:(1) Kunming Botanic Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China;(2) The Graduate School of Chinese Academy of Sciences, Beijing, China;(3) Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China
Abstract:The natural hybridization that occurs between two sympatric species of Rhododendron subgenus Hymenanthes in Yunnan, China, was investigated. The assumed parents, Rhododendron delavayi Franch. and R. decorum Franch., are morphologically distinct, and the putative hybrid species, R. agastum Balf. f. et W. W. Smith, has an intermediate morphology. We used the main morphological characters, sequences of the nuclear ribosomal DNA ITS region, and the chloroplast DNA trnL-F intronspacer to analyze the three species, and compared these morphological and molecular data with an artificial hybrid between R. decorum (♀) × R. delavayi (♂). From the results, we conclude that R. agastum is a natural hybrid between a female R. delavayi and a male R. decorum.
Keywords:ITS  Morphology  Natural hybridization            Rhododendron                      trnL-F
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