An alpha class glutathione S-transferase from pike liver |
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| Authors: | E V Borvinskaya L P Smirnov N N Nemova |
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| Institution: | 1. Institute of Biology of the Karelian Research Center of the Russian Academy of Sciences, ul. Pushkinskaya 11, Petrozavodsk, 185910, Russia
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| Abstract: | Glutathione S-transferase (GST) was isolated from the Esox lucius liver and purified to the homogenous state by SDS-PAGE and isoelectrofocusing. It was found to be a homodimer with a subunit molecular weight of 25235.36 Da (HPLC-MS/MS data) and pI of about 6.4. Its substrate specificity, thermal stability, some kinetic characteristics, and optimum pH were studied. The enzyme was identified as Alpha class GST. |
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