Tat-mediated protein delivery can facilitate MHC class I presentation of antigens |
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Authors: | Pamela Moy Yasmin Daikh Blake Pepinsky David Thomas Steve Fawell James Barsoum |
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Affiliation: | (1) Biogen Inc., 14 Cambridge Center, 02142 Cambridge, MA |
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Abstract: | We have previously shown that the tat protein of HIV-1 can be used as a carrier to promote the intracellular delivery of heterologous proteins. Here we have tested if the tat-delivery technology can be used to direct MHC class I presentation of native protein, using ovalbumin (OVA) as a model system. We show that a tat-ovalbumin conjugate (tatOVA) can be delivered into cells and that subsequent processing and presentation occurs, resulting in effective and specific killing of these target cells by an OVA specific cytotoxic T-lymphocyte (CTL) line. Comparison with the E.G7 line that expresses the OVA gene indicates that tat-mediated delivery is as efficient as endogenous expression in this system. Tat-mediated antigenic protein delivery may be useful both as a research technique and, potentially, as a therapeutic or prophylactic vaccine. |
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Keywords: | MHC class I presentation tat protein delivery ovalbumin chloroquine cytotoxic T lymphocytes |
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