R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1) |
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| Authors: | Ei-ichi Takahashi Tada-aki Hori Peter O'Connell Mark Leppert Ray White |
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| Institution: | (1) Division of Genetics, National Institute of Radiological Sciences, 4-9-1 Anagawa, 260 Chiba, Japan;(2) Howard Hughes Medical Institute, University of Utah Medical Center, 603 Wintrobe Building, 84132 Salt Lake City, UT, USA |
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| Abstract: | Summary A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidinesynchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q13.11–q13.12 in this manner, to illustrate the potential of the technique for improving the precision of chromosomal mapping and physical ordering of genes. |
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