Amplified Fragment Length Polymorphisms (AFLPs) detected with non-radioactive digoxigenine labelled primers in three plant species |
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| Authors: | Vrieling Klaas Peters Jenny Sandbrink Hans |
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| Affiliation: | (1) Van der Klaauw Laboratory, Institute of Evolutionary and Ecological Sciences, PO Box 9516, 2300 RA Leiden, The Netherlands;(2) DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, NL-6700 AA Wageningen, The Netherlands |
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| Abstract: | A protocol for the detection of AFLPs with the non radioactive digoxigenine labelling is presented. The protocol has been tested on DNA samples from three different plant species. The AFLP technique was used for the first time in these species. The sensitivity and reliability of the digoxigenine labelled primers in the AFLP technique was of the same order as the sensitivity and reliability of the radioactive assay. No major adjustments of the current standard AFLP protocols is necessary to use the digoxigenine labelled primers. |
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| Keywords: | AFLP digoxigenine labelling non-radioactive labelling |
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