|
|||||
|
|
| 维甲酸对A549细胞增殖和凋亡及Skp2、p27^kip1蛋白表达的影响 | |
| 引用本文： | 曾艳,袁静萍,毛永荣,朱润庆. 维甲酸对A549细胞增殖和凋亡及Skp2、p27^kip1蛋白表达的影响[J]. 中国组织化学与细胞化学杂志, 2009, 18(3): 322-327. DOI: 10.3870/zgzzhx.2009.03.017 |
| 作者姓名： | 曾艳 袁静萍 毛永荣 朱润庆 |
| 作者单位： | 1. 武汉市中心医院病理科,武汉,430014 2. 湖北省肿瘤医院病理科,武汉,430071 3. 武汉大学医学院病理学教研室,武汉,430071 |
| 摘 要： | 目的探讨维甲酸对A549细胞增殖和凋亡及相关基因表达的影响。方法MTT法观察ATRA对A549细胞增殖的抑制作用;流式细胞仪、AO/EB荧光双染法检测细胞凋亡;免疫细胞化学检测ATRA处理前后A549细胞Skp2、p27^kip1蛋白表达的情况。结果ATRA处理后①MTT法结果显示ATRA对A549细胞具有增殖抑制作用,在一定范围内呈时间-剂量依赖性。②AO/EB荧光双染色法观察到ATRA 25μmol/L作用A549细胞48h后,即可发现典型的凋亡形态学改变。③流式细胞仪结果出现凋亡峰,与对照组细胞相比,实验组细胞周期延长,主要表现为G0/G1期细胞比例增加,同时S期细胞比例减少。④免疫细胞化学结果显示,ATRA 25μmol/L处理细胞48h后,维甲酸处理组Skp2有明显下调,p27^kip1则明显上调。结论ATRA具有抑制肺腺癌A549细胞增殖,诱导细胞凋亡的作用,其机制可能与下调Skp2,上调p27^kip1蛋白的表达水平有关。 |
| 关 键 词： | 全反式维甲酸 A549细胞 凋亡 Skp2（细胞S期激酶相关蛋白） p27^kip1蛋白 免疫组织化学 |
EFFECT OF RETINOIC ACID ON PROLIFERATION,APOPTOSIS AS WELL AS SKP2 AND p27~(kip1) PROTEIN EXPRESSION IN A549 CELL LINE |
|
| Zeng Yan,Yuan Jingping,Mao Yongrong,Zhu Runqing. EFFECT OF RETINOIC ACID ON PROLIFERATION,APOPTOSIS AS WELL AS SKP2 AND p27~(kip1) PROTEIN EXPRESSION IN A549 CELL LINE[J]. Chinese Journal of Histochemistry and Cytochemistry, 2009, 18(3): 322-327. DOI: 10.3870/zgzzhx.2009.03.017 | |
| Authors: | Zeng Yan Yuan Jingping Mao Yongrong Zhu Runqing |
| Affiliation: | Zeng Yan,Yuan Jingping,Mao Yongrong1,Zhu Runqing2(Department of Pathology,The Central Hospital of Wuhan,Wuhan 430014,1Department ofPathology,Hubei Cancer Hospital,Wuhan 430071,2Department of Pathology,Wuhan University Medical School,China) |
| Abstract: | Objective To study the effect of retinoic acid on proliferation,apoptosis and associated gene expression of the A549 cell line.Methods The effects of ATRA on the proliferation of lung adenocarcinoma cell line A549 were determined by MTT assay.Cell apoptosis was studied by flow cytometry and acridine orange and ethidium bromide staining methods.The changes of Skp2 and p27kip1 in protein level were quantified by immunocytochemistry.Results MTT showed that ATRA had an inhibitory effect on the proliferation of ... |
| Keywords: | All-trans retinoic acid(ATRA) A549 cell Apoptosis S-phase kinase-associated protein 2(Skp2) p27kip1 protein Immunohistochemistry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |