Stimulation of the activities of solubilized pig lymphocyte RNA polymerases by phytohaemagglutinin |
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Authors: | A Cooke M Brown |
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Affiliation: | School of Biological Sciences, University of Sussex Brighton, England |
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Abstract: | DNA dependent RNA polymerase has been solubilised from pig peripheral blood lymphocytes. Using α amanitin, an inhibitor of the nucleoplasmic polymerase B activity, we have found that 20 hrs following lymphocyte stimulation with phytohaemagglutinin (PHA) the activities of both polymerase A and polymerase B are increased. As previously observed with intact nuclei a greater stimulation of polymerase A activity is observed at this time. Since the activity of these enzymes was assayed using exogenous template this indicates that PHA stimulates RNA synthesis by regulating the amounts and/or the activities of the polymerases. |
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