Morphohistological analysis and histochemistry of Feijoa sellowiana somatic embryogenesis |
| |
Authors: | G. C. Cangahuala-Inocente N. Steiner M. Santos M. P. Guerra |
| |
Affiliation: | (1) Grupo de Pesquisas em Recursos Geneticos Vegetais, Departamento de Fitotecnia, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina |
| |
Abstract: | Summary. Morphohistological analysis and histochemical studies were carried out during the induction and development of Feijoa sellowiana somatic embryos. Zygotic embryos were cultured on LPm medium containing 2,4-dichlorophenoxyacetic acid (20µM) and glutamine (8mM). Somatic embryogenesis could be induced from embryogenic cells that originated in meristematic centers or from clusters of cells. The presence of few starch grains and abundant protein bodies was observed in the globular and early torpedo stages, while in torpedo and cotyledonary-stage somatic embryos an enhanced synthesis of starch grains was associated with the accumulation of reserves to be used in the conversion of the embryos to plantlets. Proteins were predominantly observed in protoderm cells, as well as in the meristematic apical region of torpedo and cotyledonary-stage somatic embryos.Correspondence and reprints: Departamento de Fitotecnia, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, C.P. 476, 88034-001 Florianópolis, SC, Brazil. |
| |
Keywords: | : Pineapple guava Acca sellowiana Feijoa sellowiana Histological analysis Somatic embryogenesis Protein body Starch grain. |
本文献已被 PubMed SpringerLink 等数据库收录! |
|