Microbody of n-alkane-grown yeast |
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| Authors: | Susumu Kawamoto Atsuo Tanaka Midori Yamamura Yutaka Teranishi Saburo Fukui |
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| Affiliation: | (1) Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, Kyoto, Japan;(2) Department of Biology, Japan Women's University, Bunkyo-ku, Tokyo, Japan |
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| Abstract: | Microbodies appearing abundantly in n-alkane-grown cells of Candida tropicalis pK 233 were isolated by means of sucrose density gradient centrifugation. Electron microscopical observation showed that the microbodies isolated were intact. Localization of catalase and d-amino acid oxidase in the isolated microbodies was confirmed. Isocitrate lyase, malate synthase and NADP-linked isocitrate dehydrogenase were also located in the microbody, but malate dehydrogenase, citrate synthase, aconitase and NAD-linked isocitrate dehydrogenase were not. Neither cytochrome P-450 nor NADPH-cytochrome c reductase, the components involved in the n-alkane hydroxylation system of the yeast, were detected in the microbody fraction. |
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| Keywords: | Candida tropicalis Utilization of n-alkane Isolation of microbody Catalase Glyoxylate cycle |
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