地塞米松对大白鼠海马兴奋性神经元和抑制性神经元的影响

为了探讨糖皮质激素对海马兴奋性神经元和抑制性神经元的作用，本实验将地塞米松注入大白鼠侧脑室，２ｈ 后经Ｎｉｓｓｌ染色法、免疫组织化学方法和细胞计数法观察了海马谷氨酸免疫反应性（ＧｌｕＩＲ）神经元和γ氨基丁酸免疫反应性（ＧＡＢＡＩＲ）神经元的变化。结果显示：（１）ＣＡ１、ＣＡ３ 和ＳＧ区的ＧｌｕＩＲ神经元明显增多，特别是ＣＡ１ 区。经细胞计数统计分析表明，与对照组相比ＣＡ１ 有极显著性差异（Ｐ＜ ０００１），ＣＡ３区有显著性差异（００１＜ Ｐ＜ ００５），ＳＧ处无明显差异（Ｐ＞ ００５）。（２）与对照组相比，ＧＡＢＡＩＲ神经元无明显变化。结果表明，糖皮质激素有增加海马谷氨酸能神经元的作用。尽管γ氨基丁酸能神经元无明显变化，并不表明糖皮质激素对其无影响

EFFECTS OF DEXAMETHASON ON EXCITATORY AND INHIBITIVE NEURONS IN THE RAT HIPPOCAMPUS

In order to detect the effects of glucocorticoid on excitatory and inhibitive neurons in the hippocampus, dexamethason (Dex.) was injected into the rat's lateral ventricle. At 2hrs after injection the changes of glutamate (Glu IR) and gamma aminobutyric acid immunoreactive (GABA IR) neurons in the hippocampus were immunocytochemically examined. The results are as follows: (1) Glu IR neurons increased in CA1, CA3 and SG, especially in CA1. (2) GABA IR neurons did not show marked change. These results indicated that glucocorticoid had the effect of increasing Glu IR neurons in hippocampus. It did not mean that glucocorticoid had no effect on GABA IR neurons, though GABA IR neurons did not show marked change.