Molecular weight characterization of high molecular weight dextran with multiangle light scattering in on‐line and off‐line mode |
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Authors: | Jinfeng Wang Jing Wang |
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Affiliation: | Nalco, An Ecolab Company, Naperville, IL |
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Abstract: | This work reports the molecular weight (MW) analysis of high MW dextran using multiangle light scattering (MALS) in both chromatography and automated batch measurement mode. The results show that the chromatographic columns alter the high MW native dextran and cause underestimation of the MW as a consequence. Alternatively, a batch MALS measurement (without columns) provides more accurate MW values. The batch MALS measurement was automated with the incorporation of an automatic sample dilution and injection device. This automation reduces the sample preparation time and minimizes concentration errors introduced by manual sample dilution. To the best of our knowledge, this is the first study using an automated batch MALS in the analysis of high MW dextran. © 2015 Wiley Periodicals, Inc. Biopolymers 103: 387–392, 2015. |
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Keywords: | molecular weight multiangle light scattering dextran size exclusion chromatography ultrahigh molecular weight |
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