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Production of active glycosylation‐deficient γ‐secretase complex for crystallization studies |
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| Authors: | Andrés Ricardo López Mitko Dimitrov Hermeto Gerber Virginie Braman David L. Hacker Florian M. Wurm Patrick C. Fraering |
| Affiliation: | 1. Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland;2. Laboratory for Cellular Biotechnology, Institute of Bioengineering and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland;3. +41.21.693.96.51+41.21.693.95.72 |
| Abstract: | |
| Keywords: | γ ‐secretase membrane‐embedded multi‐protein complexes Alzheimer's disease glycosylation‐deficient CHO cells transposon‐mediated stable transfection protein expression and purification |