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Integrated cell and process engineering for improved transient production of a “difficult‐to‐express“ fusion protein by CHO cells
Authors:Yusuf B. Johari  Scott D. Estes  Christina S. Alves  Marty S. Sinacore  David C. James
Affiliation:1. Department of Chemical and Biological Engineering, University of Sheffield, ChELSI Institute, Mappin Street, Sheffield, UK;2. Cell Culture Development, Biogen Idec, Inc., Cambridge, Massachusetts;3. +44‐114‐222‐7505+44‐114‐222‐7501
Abstract:
Keywords:Chinese hamster ovary cells  difficult‐to‐express proteins  aggregation  unfolded protein response  cell engineering  chemical chaperones
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