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Implementing bacterial acid resistance into cell‐free protein synthesis for buffer‐free expression and screening of enzymes
Authors:Ho‐Cheol Kim  Kwang‐Soo Kim  Taek‐Jin Kang  Jong Hyun Choi  Jae Jun Song  Yun Hee Choi  Byung‐Gee Kim  Dong‐Myung Kim
Affiliation:1. Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, Korea;2. Department of Chemical and Biochemical Engineering, Dongguk University, Seoul, Korea;3. Industrial Microbiology & Bioprocess Research Center, Integrated Biorefinery Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeonbuk, Korea;4. Interdisciplinary Program for Biochemical Engineering and Biotechnology and School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea;5. +82‐42‐821‐5899+82‐42‐822‐6637
Abstract:
Keywords:cell‐free protein synthesis  glutamate decarboxylase  high‐throughput expression and screening  α  ‐2,3‐sialyltransferase  pH homeostasis
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