Engineering desiccation tolerance in Escherichia coli |
| |
| Authors: | Billi D Wright D J Helm R F Prickett T Potts M Crowe J H |
| |
| Institution: | Virginia Tech Center for Genomics (VIGEN), Fralin Biotechnology Center, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA. |
| |
| Abstract: | Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoxide. The phase transition temperatures and vibration frequencies (P==O stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
