Three-dimensional cellular ultrastructure resolved by X-ray microscopy |
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Authors: | Schneider Gerd Guttmann Peter Heim Stefan Rehbein Stefan Mueller Florian Nagashima Kunio Heymann J Bernard Müller Waltraud G McNally James G |
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Institution: | Helmholtz Zentrum Berlin für Materialien und Energie GmbH, Wilhelm-Conrad-R?ntgen Campus, Berlin, Germany. |
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Abstract: | We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ~36-nm (Rayleigh) and ~70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions. |
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