Determination of intermediates, products and cleavage site in the reaction between plasminogen activator inhibitor type-2 and urokinases |
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Authors: | D Findik P Presek |
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Affiliation: | Rudolf Buchheim-Institut für Pharmakologie, Justus Liebig-Universit?t, Giessen, FRG. |
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Abstract: | Several specific inhibitors for plasminogen activators have been isolated from various organs and cell lines, those from human placenta and the human monocyte-like cell line U-937 being virtually identical. The reaction between this type of inhibitor, designated as type-2, and high-Mr and low-Mr urokinase-type plasminogen activators was followed by reversed-phase high-performance liquid chromatography and gel electrophoresis. The components, their stable complexes and their dissociation and cleavage products could be clearly identified in both systems. The amino acid sequence of the inhibitor at the cleavage site was determined to be -Met-Thr-Gly-Arg↓Thr-Gly-His-Gly-. A 35-residue carboxy-terminal fragment was found to be released. |
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Keywords: | Plasminogen activator inhibitor Plasminogen activator Serpin Urokinase HPLC Amino acid sequence |
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