| 用DAPI染色法观察红鲫胚胎发育 |
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| 引用本文： | 张纯,刘少军.用DAPI染色法观察红鲫胚胎发育[J].激光生物学报,2011,20(4):553-555. |
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| 作者姓名： | 张纯 刘少军 |
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| 作者单位： | 教育部蛋白质化学与鱼类发育生物学重点实验室,湖南师范大学生命科学学院,长沙410081 |
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| 基金项目： | 国家自然科学基金青年项目(30901100); 湖南省教育厅科学研究项目(09B060); 国家公益性行业科研专项资金(200903046) |
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| 摘 要： | 以红鲫为研究材料,采用DAPI荧光染料对脱膜的红鲫胚胎进行染色,在荧光体视显微镜下观察红鲫胚胎早期卵裂到囊胚发育的过程.结果表明,该染色方法操作便捷,能更清晰的观察到胚胎发育的外形.值得一提的是:该方法较光学显微镜观察能清晰的观察到核DNA在细胞中的定位,如囊胚期能观察到早期卵裂细胞正进行频繁的有丝分裂等.该方法的获得...
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| 关 键 词： | DAPI 胚胎发育 荧光体视显微镜 |
The Early Embryonic Development of Red Crucian Carp Stained With DAPI |
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| ZHANG Chun,LIU Shaojun.The Early Embryonic Development of Red Crucian Carp Stained With DAPI[J].ACTA Laser Biology Sinica,2011,20(4):553-555. |
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| Authors: | ZHANG Chun LIU Shaojun |
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| Institution: | ZHANG Chun,LIU Shaojun~* (Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education of China,College of Life Sciences,Hunan Normal University,Changsha 410081,Hunan,China) |
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| Abstract: | Using fluorescence stered microscope,the early embryonic development of red crucian carp stained with DAPI is described in the present study.The results show that this method is convenient and can make the figures of embryo clearer.It is worth mentioning that using this method,the positioning of DNA in nuclei can be observed clearly.For example, the blastocysts cells showing mitosis frequently was observed by this method.The method provides a meaningful way for the study of early embryonic development of fi... |
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| Keywords: | DAPI embryonic development fluorescence stered microscope |
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