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| T-2毒素对于猪肝细胞毒性的分子机制研究 | |
| 引用本文： | 王敏辉,李吕木,钱坤,许发芝,丁小玲,刘海进.T-2毒素对于猪肝细胞毒性的分子机制研究[J].激光生物学报,2011,20(5):639-644. |
| 作者姓名： | 王敏辉 李吕木 钱坤 许发芝 丁小玲 刘海进 |
| 作者单位： | 1. 安徽农业大学动物科技学院,安徽合肥,230036 2. 安徽省畜牧生物工程技术研究中心,安徽合肥,230031 3. 安徽农业大学动物科技学院,安徽合肥230036;安徽省畜牧生物工程技术研究中心,安徽合肥230031 |
| 基金项目： | 国家“973”计划项目(2009CB118800) |
| 摘 要： | 为了探讨T-2毒素对于家畜的毒性机理及诱导凋亡的分子机制,用T-2毒素对猪的肝脏细胞进行暴露,暴露浓度分别为0、0.02mg/L、0.05mg/L、0.1mg/L,暴露时间为48h,然后用微阵列技术检测0.05mg/L处理组基因表达谱的变化。结果表明,氧化应激和细胞凋亡相关基因发生上调,药物和脂质代谢的基因发生下调,同时荧光定量PCR对细胞色素P450家族的CYP2D25和CYP51两个药物代谢基因的表达情况进行了进一步的验证。结果表明,T-2毒素对猪肝细胞的毒性机制可能是引起丝裂原活化蛋白激酶(MAPK)途径氧化应激,进而导致细胞凋亡。 |
| 关 键 词： | T-2毒素 猪 肝脏细胞 凋亡 微阵列 |
The Molecular Mechanism of T-2 Toxin-induced Toxicity in Liver Cell of Pig |
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| WANG Minhui,LI Lümu,QIAN Kun,XU Fazhi,DING Xiaoling,LIU Haijin.The Molecular Mechanism of T-2 Toxin-induced Toxicity in Liver Cell of Pig[J].ACTA Laser Biology Sinica,2011,20(5):639-644. | |
| Authors: | WANG Minhui LI Lümu QIAN Kun XU Fazhi DING Xiaoling LIU Haijin |
| Institution: | WANG Minhui~1,LI L(u|¨)mu~(1,2*),QIAN Kun~2,XU Fazhi~(1,2),DING Xiaoling~(1,LIU Haijin~1 (1.College of Animal Science and Technology,Anhui Agriculture University,Hefei 230036,Anhui,China,2.Anhui Animal Biological Engineering Technology Research Center,Hefei 230031,China) |
| Abstract: | To determine the molecular targets and toxicity mechanisms of T-2 toxin induced apoptosis in liver cell of pig.The liver cell of pig were treated with 0,0.02 mg/L,0.05 mg/L,0.1 mg/L of T-2 toxin for 48 h.To examine the gene expression profiles,the live treated with 0.05 mg/L of T-2 toxin were performed with microarray analysis.Increased expression of oxidative stress and apoptosis-related genes were detected in the liver of pig,and decreased expression of lipid metabolism- and drug- metabolizing enzyme-rela... |
| Keywords: | T-2 toxin pig liver cell apoptosis micioarray |
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