| 甜叶菊RAPD反应体系的优化 |
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| 引用本文： | 胡能兵,何克勤,张子学,林平,隋益虎,崔广荣. 甜叶菊RAPD反应体系的优化[J]. 激光生物学报, 2011, 20(4): 560-563,477. DOI: 10.3969/j.issn.1007-7146.2011.04.025 |
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| 作者姓名： | 胡能兵 何克勤 张子学 林平 隋益虎 崔广荣 |
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| 作者单位： | 安徽科技学院植物科学学院,安徽凤阳233100;安徽省甜叶菊工程技术研究中心,安徽蚌埠233000 |
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| 基金项目： | 安徽省教育厅省级重点自然科学基金项目(KJ2010A073); 蚌埠市科技计划项目(科字051) |
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| 摘 要： | 以甜叶菊为试材,对影响其RAPD反应体系的7个因子进行优化.结果表明,20 μL的优化体系包括:双蒸水13.6 μL,10×Buffer(含15 mmol/L MgCI2)溶液3μL,2.5 mmol/L的dNTPS 1.2 μL,10 μmol/L的引物1μL,20 ng的模板DNA 1μL,1UTaq聚合酶；热循环...
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| 关 键 词： | 甜叶菊 RAPD 优化 |
Optimization of'RAPD Reaction System in Stevia rebaudiana Bertoni |
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| HU Nengbing,HE Keqin,ZHANG Zixue,LIN Ping,SUI Yihu,CUI Guangrong. Optimization of'RAPD Reaction System in Stevia rebaudiana Bertoni[J]. ACTA Laser Biology Sinica, 2011, 20(4): 560-563,477. DOI: 10.3969/j.issn.1007-7146.2011.04.025 |
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| Authors: | HU Nengbing HE Keqin ZHANG Zixue LIN Ping SUI Yihu CUI Guangrong |
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| Affiliation: | HU Nengbing,HE Keqin,ZHANG Zixue,LIN Ping,SUI Yihu,CUI Guangrong~* (1.College of Plant Science,Anhui Science and Technology University,Fengyang 233100,Anhui,China,2.Anhui Province Research and Technology Center of Stevia rebaudiana Bertoni,Bengbu 233000,China) |
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| Abstract: | Optimization of 7 factors of RAPD reaction system in Stevia rebaudiana Bertoni was studied. The results in- dicated that, optimization system of 20 ~xL included：l 3.6 /μL of ddH20, 3 μL of 10 x Buffer with 15 mmol/L MgC12, 1.2 μL of 2.5 mmol/L dNTPS, 1 μL of 10 p~mol/L Primer, 1 μL of 20 ng DNA and 1 unit ofTaq polymerase; thermal cycle program was ： 1 cycle of denaturing for 4 min at 94 °C, 40 cycles of denaturing for 30 s at 94 °C, annealing for 30 s at 35 °C, primer extension for 1 min at 72°C, and the last cycle of extension for 7 min at 72 °C. |
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| Keywords: | Stevia rebaudiana Bertoni RAPD optimization |
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