Screening of the occurrence of copper amine oxidases in Fabaceae plants |
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Authors: | L Luhová M ?ebela I Frébort L Zajoncová HG Faulhammer P Pe? |
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Institution: | (1) Department of Biochemistry, Faculty of Science, Palacky, University, Šlechtitelů 11, 783 71 Olomouc, Czech Republic |
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Abstract: | Aim of this work was to find the best source for obtaining high amount of copper amine oxidase (EC 1.4.3.6) that can be further
used for analytical or industrial applications. The study focused on plant enzymes, because they occur in much higher content
in the starting material than the enzymes from other sources, have higher specific activity and are also more thermostable.
Presence of the amine oxidase was tested in extracts from 4 to 7-d-old seedlings of thirty-four various Fabaceae plants. Amine
oxidases from nine selected plants were purified by general method involving ammonium sulfate fractionation, controlled heat
denaturation, and three chromatographic steps. Kinetic properties of the amine oxidases purified were tested with a wide range
of substrates and inhibitors and were found to be very similar. Best purification yield, and total and specific activities
were obtained for the enzyme from grass pea (Lathyrus sativus) throughout all purification steps. Hence, the grass pea extract
was chosen as a suitable candidate for massive production of the amine oxidase.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Lathyrus Coronilla Lens Lupinus Medicago Melilotus Onobrychis Pisum Tetragonolobus topa quinone Trigonella Vicia |
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