Characteristics of carrot plant regenerated from two cell line selected as either ionic-Al tolerant cell or Al-phosphate utilizing cells |
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| Authors: | H Koyama K Ojima T Yamaya Y Sonoda |
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| Institution: | (1) Laboratory of Plant Cell Technology, Department of Biotechnology, Faculty of Agriculture, Gifu University, 1-1 Yanagido, 501-11 Gifu, Japan;(2) Laboratory of Plant Nutrition, Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Tsutsumidori Amamiyamachi, 981 Sendai, Japan;(3) Laboratory of Cell Biochemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Tsutsumidori Amamiyamachi, 981 Sendai, Japan |
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| Abstract: | Plantlets of carrot (Daucus carota L.) were regenerated from two types of cell lines. One type was selected as ionic-Al tolerant (IAT) cells, while the second type featured Al-phosphate utilizing cells (IPG). Their tolerance characteristics were investigated. The plantlets from IAT were directly regenerated, whereas those from IPG were regenerated after somatic hybridization with wild-type cells previously inactivated with iodacetamide, because the IPG cells had completely lost the ability to regenerate naturally.The sexual progeny of IAT showed Al-tolerant properties, established by testing their root elongation in the presence of 500 µM Al ions. Most of the calli obtained from the somatic hybrids grew more rapidly than the wild-type cells when Al-phosphate was used as a sole source of phosphorus. Thus, we obtained two types of carrot plantlets, regenerated from IAT and IPG. Both possessed the tolerant characteristics as observed with the stress-selected cells. |
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| Keywords: | Al-stress cell-fusion Daucus carota stress-selection |
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