Effects of media on embryo enlargement,germination and plant development in early-ripening genotypes of Prunus grown in vitro |
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Authors: | Richard L. Emershad David W. Ramming |
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Affiliation: | (1) Agricultural Research Service, United States Department of Agriculture, 2021 South Peach Avenue, 93727 Fresno, CA, USA |
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Abstract: | Embryo culture of 5–10 mm long embryos of Prunus was investigated. The effects of various media on embryo enlargement, germination and plant formation were compared. Results show that embryos in all genotypes enlarged during stratification on any tested medium. Beneficial embryo enlargement, germination and plant development of peach and nectarine occurred when cultured on WP medium. The embryos of a plum were more responsive to C2d medium for enlargement, germination and plant development. All genotypes germinated well with a large number of embryos growing into plants on WP and C2d media. |
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Keywords: | breeding embryo culture nectarine peach plum tissue culture |
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