| 肾上腺素受体和乙酰胆碱受体在血吸虫性肝纤维化肝脏组织中的表达研究 |
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| 引用本文： | 郭燕,高啸,刘红艳,晏维,唐望先.肾上腺素受体和乙酰胆碱受体在血吸虫性肝纤维化肝脏组织中的表达研究[J].中国组织化学与细胞化学杂志,2009,18(1). |
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| 作者姓名： | 郭燕 高啸 刘红艳 晏维 唐望先 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院肝病研究所,武汉,430030 |
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| 摘 要： | 目的研究α1A-肾上腺素受体(α1A-AR)和M1型乙酰胆碱受体(mAChRM1)在日本血吸虫性肝纤维化小鼠肝脏组织中的表达情况,初步探讨神经递质受体在肝纤维化发生机制中的作用。方法清洁级昆明小鼠30只随机分为正常组(N组,10只)、模型组(M组,20只),模型组小鼠用腹部敷贴尾蚴法感染日本血吸虫制备肝纤维化模型。应用免疫组织化学、逆转录聚合酶链反应(RT-PCR)和免疫印迹(Western blot)等方法检测肝脏组织中α1A-AR和mAChRM1表达变化。结果模型组肝脏组织中α1A-AR和mAChRM1表达较正常组明显增加,其差异具有显著性统计学意义(P<0.05)。结论神经递质受体表达上调在血吸虫性肝纤维化发生发展中起一定作用。
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| 关 键 词： | 日本血吸虫 肝纤维化 肾上腺素受体 乙酰胆碱受体 肝星状细胞 神经递质 |
EXPRESSION OF ADRENALIN RECEPTOR AND ACETYLCHOLINE RECEPTOR IN FIBROTIC LIVER TISSUE INFECTED WITH SCHISTOSOMA JAPONICUM |
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| Guo Yan,Gao Xiao,Liu Hongyan,Yan Wei,Tang Wangxian.EXPRESSION OF ADRENALIN RECEPTOR AND ACETYLCHOLINE RECEPTOR IN FIBROTIC LIVER TISSUE INFECTED WITH SCHISTOSOMA JAPONICUM[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(1). |
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| Authors: | Guo Yan Gao Xiao Liu Hongyan Yan Wei Tang Wangxian |
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| Institution: | Institute of Liver Disease;Tongji Hospital;Tongji Medical College;Huazhong University of Science and Technology;Wuhan 430030;China |
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| Abstract: | Objective To investigate the expression of α1A-adrenergic receptor (α1A-AR) and muscarinic acetylcholine receptor M1(mAChRM1) in liver tissue of fibrosis in mice infected with Schistosoma japonicum, and to explore the possible mechanism of neurotransmitter receptors in liver fibrogenesis.Methods Thirty mice were randomly divided into two groups: a normal group (10) and a model group (20). Model mice were infected with Schistosoma japonicum by transfering cercaria to the abdominal space. We detected the expr... |
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| Keywords: | Schistosoma japonicum Hepatic fibrosis Adrenalin receptor Acetylcholine receptor Hepatic stellate cell Neurotransmitter |
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