Real-time PCR assay for Clostridium perfringens in broiler chickens in a challenge model of necrotic enteritis |
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Authors: | Wu Shu-Biao Rodgers Nicholas Choct Mingan |
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Institution: | School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia |
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Abstract: | We compared ileal Clostridium perfringens quantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis indicated that the bacterial counts of these two methods were highly correlated (R(2) = 0.845). We suggest that real-time PCR could be a replacement of the culture method for quantifying C. perfringens in the intestinal tracts of broiler chickens. |
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