Expression of hsp90 mediates cytoprotective effects in the gastrodermis of planarians |
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Authors: | Maria Conte Maria Emilia Isolani Paolo Deri Linda Mannini Renata Batistoni |
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Institution: | 1Department of Biology, University of Pisa, Pisa, Italy ;2Dipartimento di Biologia, Unità di Biologia Cellulare e dello Sviluppo, Università di Pisa, S. S. Abetone e Brennero, 4, 56127 Pisa, Italy |
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Abstract: | Heat shock proteins (HSPs) play a crucial role in the protection of cells. In the present study, we have identified an hsp90-related gene (Djhsp90) encoding a cytosolic form of HSP90 that is primarily expressed in gastrodermis of the planarian Dugesia japonica. Djhsp90 becomes significantly induced after traumatic amputation or other stress stimuli, such as exposure to X-ray or ultraviolet radiations, heat shock, or prolonged starvation. When Djhsp90 is silenced by ribonucleic acid interference (RNAi), planarians dramatically decrease in size, becoming unable to eat, and die in a few weeks. Our results indicate that this gene plays an essential cytoprotective role in the gastrodermis of planarians and suggest that this chaperone can be involved in autophagic processes that are activated by this tissue. |
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Keywords: | Heat shock protein 90 Stress Planaria Intestine Expression RNAi |
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