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Functional analysis of the twin-arginine translocation pathway in Sodalis glossinidius, a bacterial symbiont of the tsetse fly |
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| Authors: | De Vooght Linda Caljon Guy Coosemans Marc Van den Abbeele Jan |
| Institution: | Department of Parasitology, Unit of Entomology, Institute of Tropical Medicine Antwerp (ITM), Antwerp, Belgium,1 Department of Animal Health, Unit of Veterinary Protozoology, Institute of Tropical Medicine Antwerp (ITM), Antwerp, Belgium,2 Unit of Cellular and Molecular Immunology, Vrije Universiteit Brussel (VUB), Brussels, Belgium,3 Department of Molecular and Cellular Interactions, VIB, Ghent, Belgium4 |
| Abstract: | This study demonstrates a functional twin-arginine (Tat) translocation pathway present in the tsetse fly symbiont Sodalis glossinidius and its potential to export active heterologous proteins to the periplasm. Functionality was demonstrated using green fluorescent protein (GFP) fused to the Tat signal peptide of Escherichia coli trimethylamine N-oxide reductase (TorA). |
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