Formation of multilayer rosettes in phytohemagglutinin-stimulated lymphocytes: correlation with chromatin condensation conformation in premature chromosome condensation |
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| Authors: | H Thomou C Sambani P Kitsiou G Politis |
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| Institution: | (1) Department of Biology,  Demokritos National Research Center of Physical Sciences, 153 10 Aghia Paraskevi Attikis, Athens, Greece;(2) Health Physics Department, Demokritos National Research Center of Physical Sciences, 153 10 Aghia Paraskevi Attikis, Athens, Greece |
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| Abstract: | We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formation suggests that changes in chromatin are a prerequisite for rosette formation. Rosette formation was most pronounced for 24-h and 48-h cultures. Chromatin decondensation and rosette formation showed identical patterns. The possibility that multilayer rosette formation is directly dependent on conformational changes of chromatin is discussed. |
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| Keywords: | T-cell stimulation PHA rosettes PCC chromatin changes |
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