| 红酵母RY-4中虾青素的分离纯化研究 |
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| 引用本文： | 郭建瑞,许激扬. 红酵母RY-4中虾青素的分离纯化研究[J]. 天然产物研究与开发, 2007, 19(5): 858-861 |
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| 作者姓名： | 郭建瑞 许激扬 |
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| 作者单位： | 中国药科大学生化教研室,南京,210009 |
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| 摘 要： | 本文主要研究了红酵母RY-4菌株中天然虾青素的提取和纯化工艺。通过各种破壁提取方法的比较分析,得出了酸热法破壁丙酮提取为较经济理想的粗色素提取方法,提取的粗色素油中虾青素含量达51.7%。通过对各种柱层析条件的摸索研究,得出了以硅胶为吸附剂,Ⅴ(石油醚)∶Ⅴ(乙酸乙酯)混和液为洗脱剂梯度洗脱从粗色素油中分离纯化虾青素的方法,所得虾青素纯度达到97%。
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| 关 键 词： | 红酵母 虾青素 破壁提取 分离纯化 柱层析 |
| 文章编号： | 1001-6880（2007）05-0858-04 |
| 收稿时间： | 2006-12-28 |
| 修稿时间： | 2007-04-09 |
Extraction and Purification of Astaxanthin from Rhodotorula RY-4 |
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| GUO Jian-rui,XU Ji-yang. Extraction and Purification of Astaxanthin from Rhodotorula RY-4[J]. Natural Product Research and Development, 2007, 19(5): 858-861 |
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| Authors: | GUO Jian-rui XU Ji-yang |
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| Abstract: | The extraction and purification of natural astaxanthin from Rhodotorula RY4 strain were studied.Various methods of breaking cell walls and extracting carotrnoids were studied.The results showed that the method of combining hydrochloric acid(HCL) with heating(by boiling water) was suitable for the cell rupturing from Rhodotorula RY-4.The method of column chromatography was adopted for the separation of astaxanthin.The immobile phase was silica-gel and the mobile phase was the mixture of V(ethyl acetate):V(petroleum ether).Then,by the method of HPLC,the results showed perfectly. |
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| Keywords: | Rhodotorula astaxanthin extraction purification CC |
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