大豆根尖维管组织分化和静止中心

用~3H-胸腺嘧啶核苷标记的自显影方法测定了大豆根尖的静止中心。结果表明在萌发后24小时产生,其高度为最大值,随着天数增加静止中心的高度逐渐减小。静止中心的高度与根的直径显著相关(r=0.94,p=0.01)。用显微光度计测定了静止中心细胞核的DNA 含量,大部分细胞在2c 水平,处在 G_1期。大豆根尖的静止中心与维管组织分化水平不相关,静止中心不直接控制维管组织的分化。

DIFFERENTIATION OF VASCULAR TISSUE AND QUIESCENT CENTRE IN THE ROOT APEX OF GLYCINE MAX(L.)MERR.

The differentiation of primary vascular tissue and the development of quiescent centre in the primary root apex of Glycine max(L.)Merr.were observed. The quiescent centre in soybean primary root originated 24 hours after germination, in size to a maximum,and studied by means of[~3H]-thymidine autoradiography.The height of quiescent centre gradually decreased with an increase of days and was cor- related with the diameter of the root(r=0.94 p=0.01).By the microdensitometer, most of the cells of quiescent centre are at the 2c level of DNA content and that are in G_1.We find that no possible direct relationship between the quiescent centre and the differentiation of vascular tissue of soybean primary root was revealed.The for- mer may not regulate the latter.