Type 1 diabetes cadaveric human pancreata exhibit a unique exocrine tissue proteomic profile |
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| Authors: | Chih‐Wei Liu Mark A Atkinson Qibin Zhang |
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| Institution: | 1. Center for Translational Biomedical Research, University of North Carolina at Greensboro, North Carolina Research Campus, Kannapolis, NC, USA;2. Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, USA;3. Department of Chemistry & Biochemistry, University of North Carolina at Greensboro, Greensboro, NC, USA |
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| Abstract: | Type 1 diabetes (T1D) is an autoimmune disorder resulting from a self‐destruction of pancreatic islet beta cells. The complete proteome of the human pancreas, where both the dysfunctional beta cells and their proximal environment co‐exist, remains unknown. Here, we used TMT10‐based isobaric labeling and multidimensional LC‐MS/MS to quantitatively profile the differences between pancreatic head region tissues from T1D (N = 5) and healthy subjects (N = 5). Among the 5357 (1% false discovery rate) confidently identified proteins, 145 showed statistically significant dysregulation between T1D and healthy subjects. The differentially expressed pancreatic proteome supports the growing notion of a potential role for exocrine pancreas involvement in T1D. This study also demonstrates the utility for this approach to analyze dysregulated proteins as a means to investigate islet biology, pancreatic pathology and T1D pathogenesis. |
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| Keywords: | Biomedicine Isobaric labeling nPOD Pancreas proteome TMT10 Type 1 diabetes |
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