Preventing N‐ and O‐formylation of proteins when incubated in concentrated formic acid |
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Authors: | Shi Zheng Alan A Doucette |
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Institution: | 1. Department of Chemistry, Dalhousie University, Halifax, NS, Canada;2. Key Laboratory of Pesticides and Chemical Biology, College of Chemistry, Central China Normal University, Wuhan, Hubei, P. R. China |
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Abstract: | Concentrated formic acid is among the most effective solvents for protein solubilization. Unfortunately, this acid also presents a risk of inducing chemical modifications thereby limiting its use in proteomics. Previous reports have supported the esterification of serine and threonine residues (O‐formylation) for peptides incubated in formic acid. However as shown here, exposure of histone H4 to 80% formic (1 h, 20oC) induces N‐formylation of two independent lysine residues. Furthermore, incubating a mixture of Escherichia coli proteins in formic acid demonstrates a clear preference toward lysine modification over reactions at serine/threonine. N‐formylation accounts for 84% of the 225 uniquely identified formylation sites. To prevent formylation, we provide a detailed investigation of reaction conditions (temperature, time, acid concentration) that define the parameters permitting the use of concentrated formic acid in a proteomics workflow for MS characterization. Proteins can be maintained in 80% formic acid for extended periods (24 h) without inducing modification, so long as the temperature is maintained at or below –20oC. |
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Keywords: | Formic acid N‐formylation O‐formylation Protein modifications Protein solubilization Technology |
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