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A mutant in Escherichia coli energy-uncoupled for lactose transporta defect in the lactose-operon |
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| Authors: | T H Wilson M Kusch E R Kashket |
| Affiliation: | 1. Laboratoire de Génomique Microbienne, Département de Sciences des Aliments, Université Laval, 2440 bl. Hochelaga, Québec, QC G1V 0A6, Canada;2. Regroupement de Recherche Pour un Lait de Qualité Optimale (Op+Lait), 3200 rue sicotte, Saint-Hyacinthe, QC J2S 2M2, Canada;3. Département de Phytologie, Université Laval, 2480 bl. Hochelaga, Québec, QC G1V 0A6, Canada;4. Food Science Department, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada;5. Département des Sciences Animales, Université Laval, 2425 rue de l''agriculture, Québec, QC G1V OA6, Canada |
| Abstract: | From a K12 strain (X71) a mutant (X71-54) has been isolated which possesses a severe defect in the active transport of thiomethyl-galactoside without reduction in total membrane carriers as assayed by o-nitrophenylgalactoside entry. Several lines of evidence suggest that the mutant is defective in the energy-coupling mechanism to the lactose transport system. This energy-uncoupled property and the lactose operon were co-transduced (via P1) into a lactose deleted strain (Ca 77). |
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