Protein trafficking rates assessed by quantum dot quenching with bromocresol green |
| |
| Authors: | Valentine Cathleen D Verkman A S Haggie Peter M |
| |
| Institution: | Department of Medicine, University of California-San Francisco, CA 94143, USA. |
| |
| Abstract: | Quantum dots are bright, photostable fluorophores used extensively to investigate biological processes. In this study, we report that bromocresol green (BCG) at low micromolar concentrations rapidly, efficiently and reversibly quenches the fluorescence of commercial quantum dots having a wide range of functionalities. The broad utility of BCG quenching of quantum dots in cell biology is showed in quantitative assays of trafficking of the β(2) -adrenergic receptor (β(2) AR) and the cystic fibrosis transmembrane conductance regulator (CFTR). |
| |
| Keywords: | β2AR CFTR fluorescence quenching protein trafficking quantum dots single‐molecule imaging |
| 本文献已被 PubMed 等数据库收录！ |
|
