Plant regeneration from somatic tissue of Rhododendron laetum x aurigeranum |
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Authors: | Giovanni Iapichino Tony H H Chen Leslie H Fuchigami |
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Institution: | (1) Department of Horticulture, Oregon State University, 97331-2911 Corvallis, OR, U.S.A.;(2) Present address: Instituto di Orticoltura e Floricoltura, Universita' di Palermo, 90100, Italy |
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Abstract: | The influence of the source of plant material (greenhouse-grown plants or in vitro shoot cultures), the type of tissue explant (shoot-tip, single-node stem segment, whole leaf, leaf strip or half-leaf section) and growth regulator concentration on shoot regeneration from somatic tissue of Rhododendron laetum × aurigeranum was evaluated. No regeneration response was obtained on explants from greenhouse-grown plants. Adventitious shoots were obtained from callus produced at the basal end of shoot-tip and single-node stem segment explants derived from in vitro-grown shoots cultured on Anderson's medium supplemented with 22.8 M IAA and 73.8 M 2iP. The greatest percentage of adventitious shoot regeneration (77%) was induced on leaf sections cultured in the presence of 22.8 M IAA and 147.6 M 2iP. Plant regeneration was accomplished with minimal callus formation. This technique represents a further step toward gene manipulation of Rhododendron.Abbreviations IAA
1-H-Indole-3-acetic acid
- 2iP
N-(3-methyl-2-Butenyl)-1H-purin-6 amine |
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Keywords: | organogenesis plant growth regulators tissue culture |
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