Endogenous protease mediated manifestation of a Ca2+-stimulated ATPase in purified dog gastric microsomes |
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Authors: | Jyotirmoy Nandi Tushar K Ray |
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Institution: | Department of Surgery, State University of New York—Upstate Medical Center, Syracuse, NY 13210 U.S.A. |
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Abstract: | An endogenous soluble protease has been demonstrated to unmask a Ca2+-stimulated ATPase activity in purified dog gastric microsomes. The presence of ATP during protease treatment appears essential for the manifestation of the gastric Ca2+-stimulated ATPase activity. The endogenous protease appears to have trypsin-like activity, since soybean trypsin inhibitor completely blocks the protease effect. Manifestation of the Ca2+-stimulated ATPase occurs without affecting the microsomal (H+ +K+)-ATPase activity and associated H+ uptake ability. The unmasked Ca2+-stimulated ATPase appears insensitive to calmodulin. Possible roles of the enzyme in the regulation of gastric H+ transport have been discussed. |
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Keywords: | ATPase Protease treatment (Dog gastric microsome) Pipes 1 4-piperazinediethanesulfonic acid |
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