Exchange between inorganic phosphate and adenosine triphosphate in (Na+,K+)-ATPase |
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| Authors: | Vera Lucia Gonçalves de Moraes Leopoldo De Meis |
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| Affiliation: | Instituto de Ciências Biomédicas, Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Cidade Universitária, Ilha do Fundão, 21.910, Rio de Janeiro, RJ Brasil |
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| Abstract: | (Na+,K+)-ATPase is able to catalyze a continuous ATP?Pi exchange in the presence of Na+ and in the absence of a transmembrane ionic gradient. At pH 7.6 the Na+ concentration required for half-maximal activity is 85 mM and at pH 5.1 it is 340 mM. In the presence of optimal Na+ concentration, the rate of exchange is maximal at pH 6.0 and varies with ADP and Pi concentration in the assay medium. ATP?Pi exchange is inhibited by K+ and by ouabain. |
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| Keywords: | Ionic gradient (Sheep kidney) |
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