Ca2+-dependent K+ transport in the Ehrlich ascites tumor cell |
| |
Authors: | Miguel Valdeolmillos Javier García-Sancho Benito Herreros |
| |
Affiliation: | Departamento de Fisiologia y Bioquímica, Facultad de medicina, Valladolid Spain |
| |
Abstract: | The possible presence and properties of the Ca2+-dependent K+ channel have been investigated in the Ehrlich ascites tumor cell. The treatment with ionophore A23187+Ca2+, propranolol or the electron donor system ascorbate-phenazine methosulphate, all of which activate that transport system in the human erythrocyte, produces in the Ehrlich cell a net loss of K+ (balanced by the uptake of Na+) and a stimulation of both the influx and the efflux of 86Rb. These effects were antagonized by quinine, a known inhibitor of the Ca2+-dependent K+ channel in other cell systems, and by the addition of EGTA to the incubation medium. Ouabain did not have an inhibitory effect. These results suggests that the Ehrlich cell possesses a Ca2+-dependent K+ channel whose characteristics are similar to those described in other cell systems. |
| |
Keywords: | Ion channel Electron donor Quinine (Ehrlich ascites cell) Hepes EGTA |
本文献已被 ScienceDirect 等数据库收录! |